Part:BBa_K2462007:Design

Sensor of halide+dehalogenase

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 786
  Illegal BglII site found at 1952
  Illegal BglII site found at 3176
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 77
  Illegal NgoMIV site found at 618
  Illegal NgoMIV site found at 1364
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 1507
  

Design Notes

We optimize the sequence of RdhANP for Bacillus megaterium.

Source

We got sensor sequence from the PKU2013 igem team part:BBa_J61051 https://parts.igem.org/Part:BBa_J61051. And we got the RdhANP sequence through tblastn of protein sequence PDB: https://www.ncbi.nlm.nih.gov/Structure/pdb/4UR3